Journal: Frontiers in Neurology
Article Title: Trench Foot or Non-Freezing Cold Injury As a Painful Vaso-Neuropathy: Clinical and Skin Biopsy Assessments
doi: 10.3389/fneur.2017.00514
Figure Lengend Snippet: Neuronal and vascular colocalisation staining in skin. Association of protein gene product 9.5 (A,B) or sensory neuron-specific receptor (C,D) neuronal staining (black), with the blood vessel endothelial cell marker cluster of differentiation 31 (red), in the subepidermis of non-freezing cold injury foot skin; scale bar = 50 µm.
Article Snippet: For immunohistochemical colocalisation studies, serial sections of human skin were double-labelled by overnight incubation with a mixture of primary polyclonal antibody to PGP 9.5 (dilution 1:40,000) or SNSR (dilution 1:15,000) and a monoclonal antibody to the vascular endothelial cell marker CD31 (Dako Cytomation, dilution 1:200).
Techniques: Staining, Marker